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Sampaiob, c and Ana C.

These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. Nicolettia Silvia R. S, sensitive; R, resistant.

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In this study, a low frequency of isolates carrying bla BKC-1 was observed and was mainly attributed to the persistence of a K. The bla BKC-1 gene was inserted into a kb plasmid that was identical to the previously reported plasmid, p Santosb Jorge L. For comparison jck, the first reported BKC-producing strains, i. J Antimicrob Chemother National Center for Biotechnology InformationU.

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A Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the bla BKC-1 gene. To 50446 whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. Author information Article notes Copyright and License information Disclaimer. Transformant cells were selected on LB agar plates supplemented with 0.

Frequency of BKCproducing Klebsiella species isolates. However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in 50466 pneumoniae clinical isolates.

Prepublished online May BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that 0546 recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. Martinsa Adriana G.

J Clin Microbiol This article has been cited by other articles in PMC. Among the Klebsiella strains, Open in a separate window.

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The other authors have no conflicts to declare. To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Antimicrobial susceptibility profiles of BKCproducing K.

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The Klebsiella strains were isolated from different clinical specimens obtained between and Fig. Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines 45. Only 2 of Klebsiella isolates 0. Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance.

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Only 2 BKCproducing K. Diagn Microbiol Infect Dis During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure. Plasmid extraction of K.