Present study illustrates the role of Fusarium oxysporum ciceri Race1 (Foc1) induced redox responsive transcripts in regulating. Abstract. Based on the differential reaction of 10 chickpea cultivars to pathogenic isolates of Fusarium oxysporum f. sp. ciceri, the existence of at. About ha are sown annually to chickpea (Cicer arietinum L.) in Andalucia, southern Spain, approximately 66% of the total national acreage of the crop.

Author: Dok Fautaur
Country: Montserrat
Language: English (Spanish)
Genre: History
Published (Last): 21 January 2006
Pages: 205
PDF File Size: 20.72 Mb
ePub File Size: 19.21 Mb
ISBN: 810-5-20209-249-5
Downloads: 46183
Price: Free* [*Free Regsitration Required]
Uploader: Gardarg

Mapping was done manually to minimize the number of evolutionary events and to infer the two simplest scenarios: In DVI, the expression was initially weak and was elevated at 7 dpi. However, in the incompatible interaction, the resistant host WR showed contrasting features. Quantitative real time polymerase chain reaction exhibited differential expression patterns of redox regulators, cellular transporters and transcription factors during Foc1 progression.

Previous studies performed on chickpea by our group as well as by other groups reported the involvement of several transcripts regulating the redox state during onset of Foc1 infection [27][29][32][36].

OCP3 appeared as f.spp.ciceri regulator of infection response, while FRO7 positively regulated iron homeostasis and photosynthesis. PLT5 plays oxyspourm role in transporting sugars primarily to non-photosynthetic tissues such as the roots [77]. The inoculated and control chickpea plants were sampled daily during 1 ff.sp.ciceri 4 DPI and at a 2—3 day interval thereafter, up to 18 DPI. Reactive oxygen species are known to play pivotal roles in pathogen perception, recognition and downstream defense signaling.

However, MYB domain containing protein showed enhanced expression in susceptible JG62 plants at all time points except for 2dpi, 4dpi and 7dpi where expression of resistant WR plants superseded that of susceptible JG62 ones. A and B represents relative expression profile of intracellular transporters like, ABC transporter like protein, carbohydrate substrate transporter, heavy metal transporter detoxifying protein FRS6translocase chloroplast 34 and polyol transporter protein in uninduced and induced JG62 and WR roots respectively; C and D represents relative expression array of cellular trafficking transporters rxces, vascular sorting receptor, clathrin coat assembly protein, secretory carrier membrane protein, nuclear pore complex protein and intrinsic protein of tonoplast in uninduced and induced JG62 and WR roots respectively; E and F shows relative expression pattern of intracellular transportation signal generators like, TRK A—N signaling factor and type II-b calcium ATPase in uninduced and induced JG62 and WR roots respectively.

  BRUNISSEMENT ENZYMATIQUE ET NON ENZYMATIQUE PDF

JG62 selection from germplasm fusairum susceptible to Fusarium wilt, while Digvijay Phule G X Bheema is resistant to the disease [ 7 ].

Races of Fusarium oxysporum f.sp. ciceri in Andalucia, southern Spain [1985]

Fow2, a Zn II 2Cys6-type transcription regulator, controls plant infection of the vascular wilt fungus Fusarium oxysporum. Results Foc1 invasion and colonization induces callose degradation, membrane disruption, and tissue damage Pathogenic events such as callose degradation, membrane disruption, and tissue damage were monitored using CSLM.

In this phase, it probably played role in plant cell wall degradation. Funding Statement This work was supported by Grant Number: Besides, this host—pathogen interplay triggered transcriptomic reprogramming directed towards regulating primary host metabolism, where sugar molecules served as defense signal modulators [32].

Earlier reports had suggested wound mediated pathogen f.sp.cicfri and HR at the site of infection [32].

Database homology matches of chickpea genes used in the study. Interestingly, PG expressed initially only in JGI and its expression decreased with the f.sp.cicerl progression and symptom development in JGI, in accordance with the results reported previously [ 11 ]. Previous reports had emphasized the role of sugars as signaling molecules [27][32].

Support Center Support Center. Values of error of biochemical assay indicating lipid fsuarium. Similarly, the expression of mitochondrial carrier protein Fow1which is responsible for the transfer of tricarboxylates across the mitochondrial inner membrane, was initially high in JGI, decreased till 4 dpi, and thereafter gradually increased reaching its peak at 28 dpi.

  HP DL320 MANUAL PDF

Interaction of Fusarium oxysporum f. sp. ciceri and Meloidogyne javanica on Cicer arietinum

Gene expression patterns support pathogen proliferation phases in the host plant Successful infection of the pathogen to the host plant requires a number of important steps like recognition and adhesion to host tissue, degradation of host tissue and resistance to host antimicrobials etc. Quantitative real time polymerase chain reaction exhibited differential expression patterns of redox regulators, cellular transporters and transcription factors during Foc1 progression.

Expression and content of sugar metabolizing genes and sugars in Foc1 infected chickpea roots. However, the efficiency of resistant cultivars in disease management is limited by pathogenic variability in pathogen populations.

American Journal of Plant Sciences. Melt curve was analyzed to evaluate primer specificity. Pathogenic events such as callose degradation, membrane disruption, and tissue damage were monitored using CSLM. However, the efficiency of resistant cultivars in disease management can be seriously limited by pathogenic variability occurring in pathogen populations, including the existence of pathogenic races defined as biotypes of a pathogen able to cause disease on host cultivars carrying specific resistance genes and pathotypes defined as biotypes of a pathogen able to cause a particular disease syndrome in the host plant.

However in DVI, initially the pathogen was restricted to root cortex region Fig 2C and 2D and reached xylem vessels very late by 18 dpi and that too in very less numbers. Strittmatter P The reaction sequence in electron transfer in the reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase system.

Received 4 December Accepted 30 January Tissue sectioning, fixation, and dehydration likely led to membrane injury resulting in abnormal stain uptake even by uninduced samples.