Evolution and Functional Diversification of Fructose Bisphosphate Aldolase have indicated particularly diverse origins of their overall gene repertoire. fructose 1,6-bisphosphate aldolases (FBAs) illustrate the influence on. Fructose-1, 6-bisphosphate aldolase (FBA) is a key plant enzyme that is Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate cloning , and evolutionary history of the chloroplast and cytosolic class I aldolases of the . Fructose-bisphosphate aldolase (EC ), often just aldolase, is an enzyme catalyzing a . “Fructose-bisphosphate aldolases: an evolutionary history”.

Author: Voodootaur Tojami
Country: Chad
Language: English (Spanish)
Genre: Health and Food
Published (Last): 4 January 2015
Pages: 24
PDF File Size: 5.38 Mb
ePub File Size: 10.52 Mb
ISBN: 405-8-82788-830-7
Downloads: 35284
Price: Free* [*Free Regsitration Required]
Uploader: Vosida

Chloroplast and cytoplasmic enzymes: Cultivating the wheat plants, sample collection, and mRNA extraction: The pfam protein families database: Therefore, analyses of gene content and derivation in chromalveolate taxa have indicated particularly diverse origins of their overall gene repertoire. The two classes share little sequence identity. Identification and characterization of a heat-induced isoform of aldolase in oat chloroplast.

Fructose-bisphosphate aldolase EC 4. We plan to conduct additional experiments to investigate the molecular mechanism underlying TaFBA biofunction.

The C- and N-termini are labeled for monomer. Sugar sensing and signaling in plants: Sign In or Create an Account. Orange circle represents Plasmodium. Owing to the transcript annotation of the wheat genome, the analysis and comparison of the structural features of the FBA s in different subfamilies aldolxses also conducted Figure 2.

Phylogenetic and structure analysis of the FBA proteins.

Fructose-1,6-bisphosphate aldolase, class 2 (IPR) < InterPro < EMBL-EBI

Approximately 23 of fructose-bsphosphate sequences were removed by pairwise comparison and the absence of complete adolase-type TIM domain. In the Calvin cycle 3-PG is produced by rubisco.


Aldolases A and C are mainly involved in glycolysiswhile aldolase B is involved in both glycolysis and gluconeogenesis. Distribution of fructose diphosphate aldolase variants in biological systems.

PCR amplification was performed as follows: Login Register Login using. Treatment of the wheat seedlings was performed as described Zeng et al. In the present study, we investigated fructose-bispohsphate responses of wheat FBA genes family to salt, drought, ABA, heat, and low temperature.

Exons are shown as Double-sided wedges, and different colours indicate different FBA gene groups. Autotrophic carbon fixation in archaea. Chuang Ma for the manuscripts review and good suggestion. The PCR results Figure 1 confirmed the location of each TaFBA gene copy, as indicated by the failed amplification in the specific nulli-tetrasomic line. We believe that whole-genome duplication and segmental duplication may have contributed to the expansion of the TaFBA gene family.

There was a problem providing the content you requested

Plants and algae have plastidal aldolase, sometimes a relic of endosymbiosisin addition to the usual cytosolic aldolase. Chinese Spring was performed.

In the present paper, 21 genes encoding TaFBA isoenzymes were identified, characterized, and categorized into three groups: Chromosomal location of TaFBA genes. Induced fit movements and metal cofactor selectivity of class II aldolases: A total of 21 TaFBA genes have been localized to 12 chromosomes of 4 homoeologous groups, and most TaFBA gene loci have three copies distributed across three genomes of wheat.

A total of 8 conserved protein domains and frkctose-bisphosphate motifs were defined Figure 3.


Untranslated regions UTRs are shown as double-sided wedges. Differentiation between homoeologous chromosomes 1A of wheat and 1Am. It and gluconeogenesis share a series of four reversible reactions. The primers used in frctose-bisphosphate study are listed in Table S2. These reactions are involved in carbon fixation and sucrose metabolism and are present in the chloroplast stroma and in the cytosol of green plants Anderson et al.

Two distinct aldolases of class II type in the cyanoplasts and in the cytosol aldolasex the alga Cyanophora paradoxa. According to the predicted protein structure, the active sites of dimeric class II FBAs also serve as the divalent metal cation-binding sites Figure 4. The thermal cycle protocol was set up as follows: In gluconeogenesis 3-PG is produced by enolase and phosphoglycerate mutase acting in series.

Exogenous calcium alleviates low night temperature stress on the photosynthetic apparatus of tomato leaves. DarkMagenta circle represents Proteobacteria.

Abiotic stimuli could rapidly trigger a significant induction of FBA genes in Sesuvium portulacastrum Fan et al.

Putative cis-acting regulatory DNA elements in the promoter sequences 1. A conserved glutamate residue exhibits multifunctional catalytic roles in D-fructose-1, 6-bisphosphate aldolases.

Distinction between cytosol historu chloroplast fructose-bisphosphate aldolases from pea, wheat, and corn leaves. The Calvin cycle is a carbon fixation pathway; it is part of photosynthesis, which convert carbon dioxide and other compounds into glucose.