Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.

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Accumulation of compatible solutes is nontoxic and does not disturb cellular functions even when they are present in high concentrations. Vertical bars represent standard error of mean anticaancer each phytochemical.

For example, the level of metabolites was increased in the leaves of salt-treated plants such as Arabidopsis thaliana [ 4 ], Oryza sativa [ panciulata ], Hordeum vulgareDatura inoxiaGlycine maxTriticum aestivum [ 5 ], and Catharanthus roseus [ 6 ].

The present study was undertaken to evaluate the anticancer activity of two aftivity plant s Andrographis paniculata and Silybum marianum aerial part and their combinational effects against five human cancer cell lines i. The analysis of variance showed that different levels of salinity affected the proline and the three main phytochemicals of A. The extracts were then stored below ambient temperature for further studies. As in cancer cells the tumor suppressor genes E-cadherin and P shows the down antidancer but while treating with the leaf extract of Andrographis paniculata the genes show up-regulation.

Likewise, many researchers suggested that proline and soluble flavonoids are involved in osmotic adjustment, protecting the cellular structures from oxidative damage and osmotic acticity by accumulation in the vacuole, and so play an important role in increasing the oxidative stress tolerance through protecting the paniculaa and photosynthetic systems versus solar radiation by absorbing UV [ 2829 ]. The germinated seeds at two initial leaf stages were transferred into the Jiffy media.

The stock solution was diluted with the same solvent to produce different concentration of working standards 0.

All chemicals used were of analytical grade. Abstract Salinity causes the adverse effects in all physiological processes of plants. The broad-sense heritability was calculated for all studied axtivity. Salinity causes the adverse effects in all physiological processes of plants. Each plant was irrigated once a day with five levels of saline water.


Phytochemical study showed the presence of flavonoids, tannins, carbohydrate, cardiac glycosides, terpenes and steroids in both the plant extracts.

View at Google Scholar K. Hepatoprotective and immunological effects of antioxidant drugs. Flavonoids isolated from Plantago species were able to strongly inhibit the proliferation of human cancer cell lines Galvez et al.

Journal of Pharmacology and Toxicology Volume 8 1: The results obtained actibity that S. Prevention of common colds with Andrographis paniculata dried extract: Among cancer especially prostrate cancer has been responsible for drastic mortality rate in males.

Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress

The combination of both the plants 1: A final drying was done by placing the concentrated extract in an electric oven at room temperature. Culturing of paniulata line: Solvents AR and chromatography grade for isolation and purification of the compounds were used as supplied by Fisher Scientific Leicestershire, UK.

Therefore, in the present study, an attempt was made to investigate the anticancer potential of hydroalcoholic extracts of A. Plant species react against the environmental changes by producing some secondary metabolites such as soluble flavonoids, which protect the cellular structures from oxidative damage anticncer osmotic stress [ 12 ].

Calibration curve was generated by linear regression based on peak areas [ 42 ]. The process of programmed cell death is called apoptosis. At high salinity level, the tolerant plants were able to tolerate the salinity stress due to the accumulation of osmoregulation and compatible solutes such as proline and soluble flavonoids, atcivity the sensitive accessions were unable to withstand the salinity stress due to the accumulation of sodium ion.

On the other hand, there was no significant correlation between the three anticancer phytochemicals and the other studied morphological traits, while correlations between AG, NAG, and DDAG were highly significant and positive Table 5.


There were strong relationships between most studied traits. There is a possibility that at the low levels of salinity, the plants might be in the adaptive process against salinity stress due to the osmotic reduction of water surrounding the root system.

In this regard, our results indicated that proline as well as the AG and NAG contents is positively correlated with the substrate concentration of salt, in which the tolerant accessions were higher in proline, AG, and NAG contents compared with the sensitive ones. Bounded stain was solubilised with Tris buffer 10 mM and the optical densities were read on an automated spectrophotometric plate reader Molecular Devices, USA at a wavelength of nm, Optical density OD of SRB in each well is directly proportional to the cell number.

Montamine, a unique dimeric indole alkaloid, from the seeds of Centaurea Montana Asteraceae and its in vitro cytotoxic activity against the colon cancer cells. PDF Kb Abstract Plant-derived compounds act as a prevention within the development of many anti-cancer agents.

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Phytochemicals, such as flavonols and flavonoids were investigated to determine chemoprevention activity against cancer Conese and Blasi, Since metabolic adaptations towards environmental stress including salinity affect global metabolic fluxes, some beneficial secondary metabolites of therapeutic importance or so-called phytochemicals would also be affected [ 11 ]. So now a days they are widely used for curing many diseases including cancer. Ten Hoopen, and J.

The comparison of andrograpyis in studied morphological traits in tolerant and sensitive accessions of A. Anticancer activity of combinational effects of A. Gour Central University, Sagar M. Reportedly, improving the content of some phytochemicals such as flavonoids as one of the most important pharmaceutical contents could be enhanced through salinity and drought stresses [ 121415 ].